TYPING CANDIDA SPP. BY THE METHOD BASED ON THE ASSESSMENT OF THE PROFILE OF THE PHENOTYPICAL SENSITIVITY / RESISTANCE TO ANTISEPTICS

Authors

  • Zh. Tsyrkunova Belarusian State Medical University
  • E. Gudkova Belarusian State Medical University
  • G. Skorokhod Belarusian State Medical University

DOI:

https://doi.org/10.31618/asj.2707-9864.2020.1.43.49

Keywords:

candidiasis, fungi, intraspecific typing, antiseptic agents, resistance, sensitivity

Abstract

The possibility of intraspecific typing the fungi of the genus Candida according to the spectrum of their sensitivity to antiseptics has been studied. The analysis of the maximum inhibitory dilution (MIR) of 16 antiseptics demonstrated the presence of both interspecies and intraspecific heterogeneity of clinical Candida isolates. The index of intraspecific heterogeneity of the studied species of the genus Candida in relation to various antiseptic agents, under experimental conditions, varied from 1 to 5. The maximum intraspecific heterogeneity of fungi was manifested in relation to the antiseptics Chlorhexidine bigluconate, hydrogen peroxide, combined antiseptics containing poly-ligexamethylene biguanidine ("Mukosanin" and "Septal"), "Hospisept Gel". According to the results of the study, an algorithm for intraspecific typing of Candida spp was proposed, based on an assessment of the phenotypic sensitivity of Candida to antiseptics. Typing of 20 clinical isolates of C.albicans was carried out. Comparative analysis of phenotypic sensitivity profiles to 4 different antiseptics made it possible to divide 20 clinical isolates of C. albicans into 12 variants, of which 6 isolates of C.albicans represented individual variants.

Author Biographies

Zh. Tsyrkunova, Belarusian State Medical University

Candidate of Biological Sciences, 

Leading Researcher, Laboratory of Nosocomial Infections

E. Gudkova , Belarusian State Medical University

Candidate of Medical Sciences, Associate Professor

G. Skorokhod , Belarusian State Medical University

Candidate of Medical Sciences, Associate Professor, 

Head of the Laboratory of Nosocomial Infections

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Published

2020-12-11

Issue

Vol. 1 No. 43 (2020)

Section

Статьи

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